|Year : 2012 | Volume
| Issue : 1 | Page : 43-46
Prevalence of methylenetetrahydrofolate reductase C677T polymorphism in eastern Uttar Pradesh
Vandana Rai, Upendra Yadav, Pradeep Kumar
Department of Biotechnology, Human Molecular Genetics Laboratory, VBS Purvanchal University, Jaunpur, Uttar Pradesh, India
|Date of Web Publication||26-May-2012|
Department of Biotechnology, VBS Purvanchal University, Jaunpur - 22001, Uttar Pradesh
| Abstract|| |
Aim: This study was aimed to evaluate the 5, 10-methylenetetrahydrofolate reductase (MTHFR) C677T mutation in eastern Uttar Pradesh population.
Materials and Methods: Polymerase chain reaction (PCR) using specific primers followed by amplicon digestion by Hinf I restriction enzyme was used for MTHFR C677T polymorphism analysis. Total 250 subjects were analyzed.
Results: The CC genotype was found in 192 subjects, followed by CT in 56 subjects and TT in 2 subject. Genotype frequencies of CC, CT and TT were 0.768, 0.224 and 0.008, respectively. The frequency of C allele was found to be 0.88 and that of T allele was 0.12.
Conclusion: It is evident from the results of the present study that the percentage of homozygous genotype (CC) is highest in the target population.
Keywords: Allele, C677T polymorphism, genotype, homocysteine, methylation, methylenetetrahydrofolate reductase, Methylenetetrahydrofolate reductase
|How to cite this article:|
Rai V, Yadav U, Kumar P. Prevalence of methylenetetrahydrofolate reductase C677T polymorphism in eastern Uttar Pradesh. Indian J Hum Genet 2012;18:43-6
|How to cite this URL:|
Rai V, Yadav U, Kumar P. Prevalence of methylenetetrahydrofolate reductase C677T polymorphism in eastern Uttar Pradesh. Indian J Hum Genet [serial online] 2012 [cited 2014 Mar 8];18:43-6. Available from: http://www.ijhg.com/text.asp?2012/18/1/43/96645
| Introduction|| |
Methylenetetrahydrofolate reductase (MTHFR) plays a central role in folate-dependent homocysteine metabolism, and severe enzyme deficiency results in elevated plasma homocysteine concentration and ultimately in syndrome homocystinuria characterized by multiple physical, developmental, and cognitive defects. Elevated serum or plasma homocysteine (tHcy) is a risk factor for a series of pathologic conditions, including cardiovascular disease,  Alzheimer disease and cognitive dysfunction,  type II diabetes,  and neural tube defects,  etc. MTHFR catalyzes the conversion of 5, 10-methylenetetrahydrofolate to 5-methylenetetrahydrofolate reductase, which donates methyl group for the conversion of homocysteine to methionine. The methyl cycle supplies 1-carbon units critical for a variety of methylation reactions essential for proper gene expression and maternal and paternal imprinting by methylated DNA.
The human MTHFR gene is 20 kb long (20,336 bp) and mapped at 1p36.3 (OMIM 607093), having 11 exons. More than 40 polymorphisms have been described in MTHFR, but the most common and clinically important variants are C677T in exon 4  and A1298C in exon 7.  The C677T variant results from a single nucleotide substitution mutation at 677 th position of the gene, in which cytosine is replaced by thymine. At protein level this change makes substitution of alanine to valine at 222nd amino acid. This change makes enzyme thermolabile with reduced enzymatic activity. This deficiency is inherited as an autosomal recessive trait. Individuals who are homozygous for the thermolabile variant of MTHFR (TT) have an increased risk of hyperhomocysteinemia and lower levels of folate in plasma and red blood cells.  C677T mutation has been reported to be a genetic factor for several disease such as neural tube defects, ,, cardiovascular diseases, , and psychiatry disorders. ,, Very limited data about MTHFR C677T mutation frequency are available for Indian population, ,,,, and no data are available on Uttar Pradesh population; hence, the aim of the present study is to estimate frequency of C677T polymorphism variant of the MTHFR gene in UP population.
| Materials and Methods|| |
Total 250 subjects between the age group of 18-70 years were randomly selected for the present study from the rural area of Jaunpur district of Uttar Pradesh. Out of which 168 were males and 82 were females. 3 ml blood samples for genetic analysis were collected in Ethylene diamine tetraacetic acid disodium salt (EDTA) -coated vials and informed consent was obtained from each subject. Only unrelated individuals participated in the study. The present study was conducted in the Human Molecular Genetics Laboratory, Department of Biotechnology, VBS Purvanchal University, Jaunpur, India, during the period 2008-2010.
Genomic DNA extraction
Genomic DNA was extracted according to the method of Bartlett and White  with slight modification. Extracted DNA was stored at - 20°C until the genotype analysis was performed.
MTHFR genotype determination
Analysis of the MTHFR C677T mutation was based on the method of Frosst et al.  Polymerase chain reaction (PCR) was performed using genomic DNA and the primers 5'-TGAAGGAGAAGGTGTCTGCGGGA-3'and 5'-AGGACGGTGCGGTGAGAGTG-3' to generate a 198-bp fragment. PCR was performed in MJ Mini thermo cycler (Bio-Rad, USA), and the program consisted of an initial melting step of 2 min at 94°C, followed by 40 cycles of 1 min denaturation at 94°C, 1 min annealing at 65°C, 1 min extension at 72°C, and a final elongation step of 10 min at 72°C. The amplified product was digested with Hinf I restriction enzyme (Genei, India), which cleaves only mutant MTHFR allele into 175- and 23-bp fragments. Amplification and restriction products were analyzed by use of electrophoresis in 2% and 4% agarose (Fermentas) gels, respectively.
| Results and Discussion|| |
Amplification with MTHFR specific primers produced 198 bp long amplicon [Figure 1]. After digestion with Hinf I wild homozygous (CC) remained uncut, and produced one band of 198 bp, homozygous mutant produced two bands (175bp and 23bp) and heterozygous (CT) genotype produced three bands (198bp, 175bp and 23 bp) in agarose gel electrophoresis [Figure 2]. Genotype distribution and allele frequencies in the present study are presented in [Table 1]. The CC genotype was found in 192 subjects (76.8%), followed by CT in 56 subjects (22.4%) and TT in 2subject (0.81%). The conformity of the genotype frequency distribution to Hardy-Weinberg proportion was examined using X2 test. Genotype frequencies of CC, CT and TT were 0.768, 0.224 and 0.008, respectively. The frequency of C allele was found to be 0.88 and that of T allele was 0.12. It is evident from the results of the present study that the percentage of homozygous genotype (CC) is highest in the target population.
|Figure 2: RFLP analysis for the C677T mutation on 198-bp MTHFR PCR products with Hinf I. Wild-type homozygous remains uncut after Hinf I digestion gives one band, mutant homozygous gives two bands (175-bp and 23-bp), and heterozygous gives three bands(198-bp,175-bp, and 23-bp).The figure shows normal CC (lanes: 2, 5, and 7), heterozygous CT (lanes: 3 and 6), and homozygous mutant TT (lane: 4) genotypes|
Click here to view
|Table 1: MTHFR genotype and allele frequency distribution among Uttar Pradesh population|
Click here to view
Ample data on the worldwide frequency of this variant are currently available. ,,,,,,,, The frequency of the MTHFR 677T allele varies substantially in different regions of the world and among ethnic groups. For example, the allele frequency ranges from 0.20 to 0.55 in Europeans and from 0.04 to 0.38 in Asian populations. ,, The T allele frequency (0.12) in our target population is fell within the range of T allele frequency (0.04 to 0.38) reported in other studies published about the Asian populations. The result of our study on MTHFR polymorphism supplements the variability of this gene worldwide and can serve as a basis for further association studies on the role of MTHFR mutation in the susceptibility of different multifactorial diseases.
| Acknowledgments|| |
We are grateful to the subjects who participated in the present study without their cooperation; this study could not be completed. The financial support from University Grants Commission, New Delhi (grant No. 32-548/2006(SR)) and Department of Biotechnology (No BT/PR98887/SPD/11/1028/2007) as major research project to Vandana Rai is gratefully acknowledged.
| References|| |
|1.||Ueland PM, Refsum H, Beresford SA, Vollset SE. The controversy over homocysteine and cardiovascular risk. Am J Clin Nutr 2000;72:324-32. |
|2.||McCaddon A, Davies G, Hudson P, Tandy S, Cattell H. Total serum homocysteine in senile dementia of Alzheimer type. Int J Geriatr Psychiatry 1998;13:235-9. |
|3.||De Luis DA, Fernandez N, Arranz ML, Aller R, Izaola O, Romeo EJ. Total homocysteine levels relation with other cardiovascular risk factors in a population of patients with diabetes mellitus type 2. J Diabetes Complications 2005;19:42-6. |
|4.||Mills JL, Mcpartlin JM, Kirke PN, Lee YJ, Conley MR, Weir DG, et al. Homocysteine metabolism in pregnancies complicated by neural tube defects. Lancet 1995;345:149-52. |
|5.||Frosst P, Blom HJ, Milos R, Goyette P, Sheppard CA Mattews RG, et al. A candidate genetic risk factor for vascular disease: A common mutation in methylenetetrahydrofolate reductase. Nat Genet 1995;10:111-3. |
|6.||Weisberg I, Tran P, Chritensen B, Sibani S, Rozen R. A second genetic polymorphism in methylentetrahydrofolate reductase (MTHFR) associated with decreased enzyme activity. Mol Genet Metab 1998;64:169-72. |
|7.||Molloy AM, Daly S, Mills JL, Kirke PN, Whitehead AS, Ramsbottom D, et al. Thermolabile variant of 5,10-methylenetetrahydrofolate reductase associated with low red-cell folate: Implications for folate intake recommendations. Lancet 1997;349:1591-3. |
|8.||Whitehead AS, Gallagher P, Mills JL, Kirke PN, Burke H, Molloy AM, et al. A genetic defect in 5,10-methylenetetrahydrofolate reductase in neural tube defects. Q J Med 1995;88:763-6. |
|9.||Van der Put NM, Eskes TK, Blom HJ. Is the common 677CT mutation in the methylenetetrahydrofolate reductase gene a risk factor for neural tube defects? A meta-analysis. Q J Med 1997;90:111-5. |
|10.||Brilakis ES, Berger PB, Ballman KV, Rozen R. Methylenetetrahydrofolate reductase (MTHFR) 677C>T and methionine synthase reductase (MTRR) 66A>G polymorphism association with serum homocysteine and angiographic coronary artery disease in the era of flour product fortified with folic acid. Atherosclerosis 2003;168:315-22. |
|11.||Tan EC, Chong SA, Lim LC, Chan AO, Teo YY, Tan CH, et al. Genetic analysis of the thermolabile methylenetetrahydrofolate reductase variant in schizophrenia and mood disorder. Psychiatr Genet 2004;14:227-31. |
|12.||Chen CS, Tsai JC, Tsang HY, Kuo YT, Lin HF, Chiang IC, et al. Homocysteine levels, MTHFR C677T genotype, and MRI hyperintensities in late onset major depressive disorder. Am J Geriatr Psychiatry 2005;13:869-75. |
|13.||Sazci A, Ergul E, Kucukali I, Kara I, Kaya G. Association of the C677T and A1298C polymorphisms of methylenetetrahydrofolate reductase gene with schizophrenia: Association is significant in men but not in women. Prog Neuropsychopharmacol Biol Psychiatry 2005;29:1113-23. |
|14.||Mukherjee M, Joshi S, Bagadi S, Dalvi M, Shetty KR. A low prevalence of the C677T mutation in the methylenetetrahydrofolate reductase gene in Asian Indian. Clin Genet 2002;61:155-9. |
|15.||Radha Rama Devi A, Govindaiah V, Ramakrishna G, Naushad SM. Prevalence of methylenetetrahydrofolate reductase gene polymorphism in south Indian population. Curr Sci 2004;86:440-3. |
|16.||Mukhopadhyay K, Dutta S, Das Bhomik A. MTHFR gene polymorphisms analyzed in population from Kolkata, West Bengal. Indian J Hum Genet 2007;13:38. |
|17.||Bhat TA, Mir MR, Qasim I, Misra SS, Kirmani MA. Genetic polymorphism of 5,10-methylenetetrahydrofolate reductase C677T in Kashmiri population. Biotechnology 2008;7:822-5. |
|18.||Saraswathy KN, Mukhopadhyay R, Sinha E, Aggarwal S, Sachdeva MP, Kalla AK. MTHFR C677T polymorphisms among the Ahirs and Jats of Haryana (India). Am J Hum Biol 2008;20:116-7. |
|19.||Bartlett JM, White A. Extraction of DNA from blood. In: Bartlett JM, Stirling D, editors. Methods in Molecular Biology. PCR Protocls. 2 nd ed., vol. 226. Totowa, NJ: Humana Press Inc.; 2003. |
|20.||Aruda VR, Siqueira LH, Goncalves MS, von Zuben PM, Soares MC, Menezes R, et al. Prevalence of the mutation C677T in methylenetetrahydrofolate reductase gene among distinct ethnic groups in Brazil. Am J Med Genet 1998;78:332-5. |
|21.||Giles WH, Kittner SJ, Ou CY, Croft JB, Brown V, Buchholz DW, et al. Thermolabile methylenetetrahydrofolate reductase polymorphism (C677T) and total homocysteine concentration among African, American and White women. Ethn Dis 1998;8:149-57. |
|22.||Rady PL, Szues S, Grady J, Hudnall SD, Kellner LH, Nitowsky H, et al. Genetic polymorphisms of methylenetetrahydrofolate reductase (MTHFR) and methionine synthase reductase (MTRR) in ethnic populations in Texas: A report of a novel MTHFR polymorphic site, G1793A. Am J Hum Genet 2002;107:162-8. |
|23.||Rosenberg N, Murata M, Ikeda Y, Opare-Sem O, Zivelin A, Geffen E, et al. The Frequent 5,10-Methylenetetrahydrofolate Reductase C677T Polymorphism is associated with a Common Haplotype in Whites, Japanese, and Africans. Am J Hum Genet 2002;70:758-62. |
|24.||Sadewa AH, Sunarti Retno S, Hayashi C, Lee MJ, Ayaki H, Sofro AS, et al. The C677T mutation in the methylenetetrahydrofolate reductase gene among the Indonesian havanese population. Kobe J Med Sci 2002;48:137-44. |
|25.||Esfahani ST, Cogger EA, Caudill MA. Heterogeneity in the prevalence of methylenetetrahydrofolate reductase gene polymorphism in women of different ethnic groups. Am Diet Assoc 2003;103:200-7. |
|26.||Spiridonova MG, Stepanov VA, Maximova NR, Puzyrev VP. Population study of frequency of Methylenetetrahydrofolate reductase C677T gene population in Yakutia. Genetika 2004;40:570-3. |
|27.||Pepe G, Venegas OC, Giusti B, Brunelli T, Marucci R, Attanasio M, et al. Heterogeneity in world distribution of thermolabile C677T mutation in 5,10-methylenetetrahydrofolate reductase. Am J Hum Genet 1998;63:917-20. |
|28.||Schneider JA, Rees DC, Liu YT, Clegg JB. Worldwide distribution of a common methylenetetrahydrofolate reductase mutation. Am J Hum Genet 1998;62:1258-60. |
[Figure 1], [Figure 2]